[Un metodo eficiente para propagacion in vitro de rizomas de Alstroemeria pallida Graham]
Rice Breeding Program (RBP) of the Instituto de Investigaciones Agropecuarias (INIA) at Chillán, Chile, has a rice (Oryza sativa L.) germplasm collection that consists of 1200 accessions, mainly temperate japonica rice accessions, well adapted to the local conditions. Most of the new introduced accessions adapt very poorly to Chilean agroecological conditions because of requirements of long days and cold tolerance. The objectives of this study were to use microsatellites to evaluate level of polymorphism of a representative sample of this collection and determine its genetic diversity and relationships with cultivated germplasm from different geographical origin. A total of 249 genotypes were analyzed with 30 selected polymorphic microsatellites. Total number of alleles scored across 249 genotypes was 183 with an overall mean of 6.1 alleles per locus, ranging 2-14. The mean major allele (most common) frequency was 0.61 and mean minor allele frequency was 0.028. The overall mean gene diversity across 30 SSR loci was 0.52. Mean heterozygosity was 0.01, and mean polymorphism information content (PIC) value was 0.47.