Occurrence and molecular characterization of Potyvirus present in the garlic crop in the Mediterranean ecosystem of the central valley of Chile

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dc.contributor.authorMadariaga V., Mónica
dc.contributor.authorRamírez A., Isabel
dc.contributor.authorLizana, Rodrigo
dc.contributor.authorNova L., Nelly
dc.contributor.authorDonoso M., Adolfo
dc.contributor.authorTorrejón, Valentina
dc.coverageChilees_ES
dc.date.accessioned2023-12-27T21:13:05Z
dc.date.available2023-12-27T21:13:05Z
dc.date.issued2023-12
dc.description.abstractThe garlic (Allium sativum L.) cultivated in the O’Higgins Region, the main garlic-producing area in Chile, is the commercial type known as pink garlic, which presents a low yield and a high prevalence of symptoms associated with viral infections. A survey was carried out in the three localities of the region that cultivate this vegetable to identify, by reverse transcription polymerase chain reaction (RT-PCR), the presence of Onion yellow dwarf virus (OYDV) and Leek yellow stripe virus (LYSV). The results indicated a prevalence of 99.5% and 54.0% respectively. In addition, a molecular and phylogenetic analysis of the amplified genome fragments for both viruses was performed. Using the nucleotide BLAST (BLASTn) tool, nucleotide sequences were compared with sequences available in the National Center for Biotechnology Information (NCBI) database. The Chilean sequences were aligned with complete sequences available at NCBI and a phylogenetic tree was constructed using the Neighbor-Joining method. The results showed genetic variability among Chilean isolates.es_ES
dc.formatapplication/pdfes_ES
dc.identifier.urihttps://hdl.handle.net/20.500.14001/69175
dc.language.isoIngléses_ES
dc.numero.paginas10 p.es_ES
dc.placeofeditionChillán, Chilees_ES
dc.publisherInstituto de Investigaciones Agropecuariases_ES
dc.relation.ispartofChilean Journal of Agricultural Researches_ES
dc.relation.ispartofnov. 83(6) p. 705-714es_ES
dc.subject.spanishajoes_ES
dc.subject.spanishpotyviruses_ES
dc.subject.spanishpotexviruses_ES
dc.subject.spanishvirus de las plantases_ES
dc.titleOccurrence and molecular characterization of Potyvirus present in the garlic crop in the Mediterranean ecosystem of the central valley of Chilees_ES
dc.title.alternativeOcurrencia y caracterización molecular de Potyvirus presentes en el cultivo de ajo en el ecosistema mediterráneo del valle central de Chile.es_ES
dc.typeArtículo

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2.2.2. Chilean Journal (Ex- Agricultura Técnica)